The mutagenic action of N-methyl-N'-nitro-N-nitrosoguanidine on Coprinus lagopus.

Optimal conditions, defined by the use of a known auxotrophic strain, are described for the mutagenesis of Coprinus Zagopus oidia by N-methyl-N’nitro-N-nitrosoguanidine (NTG), which is a very effective mutagen for bacteria and yeast. The oidia were best treated in phosphate buffer (PH 6.8) with a concentration of 15 pg. NTG/ml. for about 70 min. The yield of auxotrophs was considerably less than might be expected from a comparison of other systems in which NTG has been used as a mutagen.